Ex (nm)
|
502
|
Em (nm)
|
522
|
分子量
|
661.17
|
溶劑
|
DMSO
|
存儲條件
|
在零下15度以下保存, 避免光照
|
Helixyte Green dsDNA染料是美國AAT Bioquest生產的一種超靈敏熒光核酸染料,用于定量溶液中的雙鏈DNA(dsDNA)。近期,Helixyte Green dsDNA染料用于PCR產物的直接循環測序方法中的PCR擴增產量。使用標準光譜熒光計和2.5 ng / mL帶有熒光酶標儀的dsDNA檢測到少至25 pg / mL的dsDNA(在2 mL測定體積中50 pg dsDNA),在ssDNA,RNA和ss存在下檢測效果小 游離核苷酸。該測定在三個數量級上是線性的并且幾乎沒有序列依賴性。它是從多種來源準確測量DNA的理想選擇,包括基因組DNA,病毒DNA,小量制備DNA或PCR。百螢生物是AAT Bioquest的中國代理商,為您提供的Helixyte Green 雙鏈DNA定量試劑
參考文獻
Inhibitors of Streptococcus pneumoniae surface endonuclease EndA discovered by high-throughput screening using a PicoGreen fluorescence assay
Authors: Peterson EJ, Kireev D, Moon AF, Midon M, Janzen WP, Pingoud A, Pedersen LC, Singleton SF.
Journal: J Biomol Screen (2013): 247
Validation of a PicoGreen-based DNA quantification integrated in an RNA extraction method for two-dimensional and three-dimensional cell cultures
Authors: Chen Y, Sonnaert M, Roberts SJ, Luyten FP, Schrooten J.
Journal: Tissue Eng Part C Methods (2012): 444
Characterization of PicoGreen interaction with dsDNA and the origin of its fluorescence enhancement upon binding
Authors: Dragan AI, Casas-Finet JR, Bishop ES, Strouse RJ, Schenerman MA, Geddes CD.
Journal: Biophys J (2010): 3010
Comparison of SYBR Green I-, PicoGreen-, and [3H]-hypoxanthine-based assays for in vitro antimalarial screening of plants from Nigerian ethnomedicine
Authors: Abiodun OO, Gbotosho GO, Ajaiyeoba EO, Happi CT, Hofer S, Wittlin S, Sowunmi A, Brun R, Oduola AM.
Journal: Parasitol Res (2010): 933
。