鈣離子熒光探針Cal-520N, AM是美國AAT Bioquest生產的用于標記鈣離子的熒光探針，Cal-520®提供強大的均勻熒光測定工具，用于檢測細胞內鈣動員。Cal-520®AM是一種新型熒光鈣敏感染料，與現有的綠色鈣指示劑（如Fluo-3 AM和Fluo-4 AM）相比，具有顯著信噪比和細胞內保留。表達通過鈣信號傳導的GPCR或鈣通道的細胞可以預裝載可以穿過細胞膜的Cal-520 AM。一旦進入細胞內，Cal-520 AM的親脂性阻斷基團被酯酶裂解，產生帶負電荷的熒光染料，留在細胞內。與鈣結合后，其熒光大大增強。當用激動劑刺激細胞時，該受體表示細胞內鈣的釋放，這顯著增加了Cal-520®的熒光。Cal-520®AM具有長波長，高靈敏度和> 100倍熒光增強的特性，是測量細胞鈣的理想指示劑。高信噪比和更好的細胞內保留使Cal-520®鈣測定成為評估GPCR和鈣通道靶標以及篩選其激動劑和拮抗劑的有力工具。百螢生物是AAT Bioquest 的中國代理商，為您提供的鈣離子熒光探針

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