CytoTrace 紅色熒光探針保留了Cy3 / TRITC的光譜特性，因此可以使用熒光顯微鏡或流式細胞儀輕松檢測其信號。它自由地穿過細胞膜，并在與細胞成分反應后轉化為不滲透細胞的產物。細胞不滲透的反應產物經過數代傳給子細胞，但沒有轉移到群體中的相鄰細胞。裝有CytoTrace Red探針的細胞通常可以發出熒光，并且至少可以存活24小時，這使該探針成為了出色的長期細胞示蹤劑。染色 圖案可以用甲醛或戊二醛固定，用于信號放大和其他應用。它的光譜與GFP或FITC標記的抗體的光譜很好分離

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